| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 541 | Leptospira biflexa | ATCTCTGGCACTGGCACTT | TTTTGGGTGCTGCCGTGT | 58.93 | 60.44 | 227 | 103 |
100.00%
|
100.00%
|
| Primer 542 | Leptospira biflexa | TGGAGTTGGTTCCGATGCA | TGGTGCGCTGTTTGGAGA | 59.24 | 59.49 | 257 | 103 |
100.00%
|
100.00%
|
| Primer 543 | Leptospira biflexa | AGGTGTTGCAAGCGGATCA | ACCACAAACGCGAACCAGA | 59.93 | 60.15 | 235 | 103 |
100.00%
|
100.00%
|
| Primer 544 | Leptospira biflexa | AGGTGTTGCAAGCGGATCA | CTGCGAGCTGGTTTCCCTT | 59.93 | 60.30 | 261 | 103 |
100.00%
|
100.00%
|
| Primer 545 | Leptospira biflexa | ATCCCCTCCAGCAGCTCTT | AACACCCATGGAACGGCA | 60.31 | 59.48 | 199 | 103 |
100.00%
|
100.00%
|
| Primer 546 | Leptospira biflexa | AAAGAAGGTGCGCTGGCT | TCCCAAGGCCAAATGCAGA | 59.89 | 59.54 | 297 | 103 |
100.00%
|
100.00%
|
| Primer 547 | Leptospira biflexa | TCCGGAGCTACCCAACCTT | ACGGCATGTTGGAAGGCT | 60.23 | 59.56 | 173 | 103 |
100.00%
|
100.00%
|
| Primer 548 | Leptospira biflexa | TGGTGACAAAGCCAATACGC | CGGCATGTTCGCTGTATGC | 59.12 | 60.01 | 155 | 103 |
100.00%
|
100.00%
|
| Primer 549 | Leptospira biflexa | TCCCAACCGTCGCAAAGA | TAGGCACCTCTCACCGGAA | 59.18 | 59.92 | 276 | 103 |
100.00%
|
100.00%
|
| Primer 550 | Leptospira biflexa | TGGCGACAACTCAGTGTAGA | CGCATCTGAGGCACTGGAT | 58.68 | 59.85 | 267 | 103 |
100.00%
|
100.00%
|